Evaluation of circulating osteopontin level as potential biomarker of allergic asthma in patients with Caucasian and South-East Asian ethnicity in Allergy: European Journal of Allergy and Clinical Immunology
2018
ASL Vercelli
Tipo pubblicazione
Conference Abstract
Autori/Collaboratori (17)Vedi tutti...
Villa E
Pneumology University Department, ASL Vercelli, Vercelli, Italy
Andiappan A
Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A STAR), Singapore, Singapore
Boggio E
Laboratory of Immunology, Department of Health Sciences, University of Eastern Piedmont, Novara, Italy
et alii...
Abstract
Background: Osteopontin (OPN) is a pleomorphic cytokine known to influence a wide range of immune cells; allergic asthma was previously associated with high circulating OPN levels. In the present study, we aimed to verify if OPN may qualify as biomarker of activated immune response in allergic patients belonging to two different ethnic groups: Caucasians and South-East Asians. Method: Serum OPN levels were measured by ELISA test (Human Osteopontin Duoset, R and D Systems) in a series of 121 Italian adult patients affected by extrinsic asthma, allergic rhinitis, Hymenoptera venom allergy, food allergy, allergic contact dermatitis and IgE mediated hypersensitivity to beta lactams. 116 healthy subjects served as controls. 576 ethnic Chinese subjects were recruited at the National University of Singapore (NUS) as cross-sectional cohort of an ongoing epidemiological study on the national prevalence of allergic diseases, and OPN levels were detected by Luminex (Milliplex Map, Merck) and ELISA assays (R and D Systems). Results: In the Italian cohort, OPN levels were significantly higher in cases compared to controls (P = 0.0010 by the Mann-Whitney test). Statistically higher OPN levels were found in asthma (P = 0.0269) and food allergy (P = 0.046) groups in comparison to controls. No significant differences were found (P = 0.597) between Singaporeans with lifetime asthma and healthy controls, only the highest OPN levels were heterogeneously found to correlate with asthma. However, a strong gender effect was shown, in both cases (P < 0.0001) and controls (P < 0.0001), with males presenting higher OPN levels in comparison to females. Consequently, we checked the mRNA expression levels of OPN gene (SPP1) with Illumina chips in whole blood of males and females, and no difference was found (P < 0.05). Several experiments with Western Blots and different gel types were performed to verify if possible post-transcriptional/post-translational modifications of OPN could expla
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DOI : 10.1111/all.13539
Keywords
beta lactam; biological marker; endogenous compound; osteopontin; adult; allergic asthma; allergic rhinitis; Caucasian; Chinese; cohort analysis; conference abstract; enzyme linked immunosorbent assay; ethnic group; ethnicity; extrinsic asthma; female; food allergy; gender; gene expression; genetic transcription; human; human cell; human tissue; immune response; Italian (citizen); major clinical study; male; mRNA expression level; prevalence; protein blood level; protein expression; protein processing; rank sum test; Singapore; Singaporean; allergic contact dermatitis; Western blotting;